Journal: Bioactive Materials

Article Title: A hierarchical MgF 2 /polyurethane/pitavastatin coating alleviates degradation and enhances endothelialization of bioresorbable magnesium alloy stents

doi: 10.1016/j.bioactmat.2025.08.038

Figure Lengend Snippet: Effects of bare and coating-modified groups on SMCs proliferation. (A–C) Representative fluorescence images of HUASMCs cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: Human umbilical vein ECs (HUVECs), human umbilical artery SMCs (HUASMCs), and MΦs were obtained from Procell Life Science & Technology Co., Ltd. All cell lines were maintained and passaged in a cell culture incubator at 37 °C with 5 % CO 2 .

Techniques: Modification, Fluorescence, Control, Expressing, Comparison

Journal: Cytotechnology

Article Title: MiR-29b mimics promotes cell apoptosis of smooth muscle cells via targeting on MMP-2

doi: 10.1007/s10616-017-0150-z

Figure Lengend Snippet: miR-29 mimics downregulated MMP-2 expression in human umbilical aortic smooth muscle cells. 48h after transfection with miR-29 mimics, the expression of miR-29 and MMP-2 was detected. a miR-29 expression by qRT-PCR. b expression of MMP-2 mRNA by qRT-PCR. c expression of MMP-2 by western blot. **p < 0.01, ***p < 0.001 versus NC

Article Snippet: Cell culture and transfection Human umbilical aortic smooth muscle cells (SMC) (Lonza, Walkersville, USA) were cultured in MCDB-131 Medium supplemented with 5% Fetal Bovine Serum, 2 mM l -Glutamine, 100 U/mL Penicillin, 0.1 mg/mL Streptomycin and 12.5 U/mL Nystatin, 2 ng/mL Insulin (Sigma Aldrich, St Louis, MO, USA), 0.5 ng/mL Epithelial Growth Factor (Sigma Aldrich), 3 ng/mL Basic-Fibroblasts Growth Factor (bFGF) (PeproTech Inc., Rocky Hill, New Jersey, USA) and 1 μg/mL Hydrocortisone (Sigma Aldrich) at 37 °C in a humidified atmosphere containing 5% CO 2 .

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot

Journal: Cytotechnology

Article Title: MiR-29b mimics promotes cell apoptosis of smooth muscle cells via targeting on MMP-2

doi: 10.1007/s10616-017-0150-z

Figure Lengend Snippet: miR-29 mimics reduced cell viability in human umbilical aortic smooth muscle cells. 48 h after transfection with miR-29 mimics, cell viability was assessed at 24, 48 and 72 h in the presence or absence of MMP-2 inhibitor SB-3CT (100 nM) by a CCK-8 assay and b Trypan blue exclusion assay. *p < 0.05; **p < 0.01 versus NC

Article Snippet: Cell culture and transfection Human umbilical aortic smooth muscle cells (SMC) (Lonza, Walkersville, USA) were cultured in MCDB-131 Medium supplemented with 5% Fetal Bovine Serum, 2 mM l -Glutamine, 100 U/mL Penicillin, 0.1 mg/mL Streptomycin and 12.5 U/mL Nystatin, 2 ng/mL Insulin (Sigma Aldrich, St Louis, MO, USA), 0.5 ng/mL Epithelial Growth Factor (Sigma Aldrich), 3 ng/mL Basic-Fibroblasts Growth Factor (bFGF) (PeproTech Inc., Rocky Hill, New Jersey, USA) and 1 μg/mL Hydrocortisone (Sigma Aldrich) at 37 °C in a humidified atmosphere containing 5% CO 2 .

Techniques: Transfection, CCK-8 Assay, Trypan Blue Exclusion Assay

Journal: Cytotechnology

Article Title: MiR-29b mimics promotes cell apoptosis of smooth muscle cells via targeting on MMP-2

doi: 10.1007/s10616-017-0150-z

Figure Lengend Snippet: miR-29 mimics enhanced cell apoptosis in human umbilical aortic smooth muscle cells. 48 h after transfection with miR-29 mimics for 48 h and in presence or absence of MMP-2 inhibitor SB-3CT (100 nM) for 10 h, the cell apoptosis was detected by flow cytometry. a Flow cytometry; b quantification of the early, late, and total apoptosis. *p < 0.05 versus NC

Article Snippet: Cell culture and transfection Human umbilical aortic smooth muscle cells (SMC) (Lonza, Walkersville, USA) were cultured in MCDB-131 Medium supplemented with 5% Fetal Bovine Serum, 2 mM l -Glutamine, 100 U/mL Penicillin, 0.1 mg/mL Streptomycin and 12.5 U/mL Nystatin, 2 ng/mL Insulin (Sigma Aldrich, St Louis, MO, USA), 0.5 ng/mL Epithelial Growth Factor (Sigma Aldrich), 3 ng/mL Basic-Fibroblasts Growth Factor (bFGF) (PeproTech Inc., Rocky Hill, New Jersey, USA) and 1 μg/mL Hydrocortisone (Sigma Aldrich) at 37 °C in a humidified atmosphere containing 5% CO 2 .

Techniques: Transfection, Flow Cytometry

Journal: International Journal of Medical Sciences

Article Title: The phenotype of vascular smooth muscle cells co-cultured with endothelial cells is modulated by PDGFR-β/IQGAP1 signaling in LPS-induced intravascular injury

doi: 10.7150/ijms.34749

Figure Lengend Snippet: ECs induced higher TNF-α expression in SMCs . (A) , The quantification of TNF-α expression in SMCs under single-culture or co-culture system. **: P<0.01, SMCs were treated with control vehicle in single-culture system (SMC Control) vs SMCs were treated with control vehicle in co-culture system (CULSMC Control), ##: P<0.01, SMCs were treated with LPS in single-culture (SMC LPS) vs SMCs were treated with LPS in co-culture (CULSMC LPS), @@: P<0.01, CULSMC Control vs CULSMC LPS; (B) , No significant difference in the level of PDGF-BB was shown in the above-mentioned groups.

Article Snippet: Human umbilical vein SMCs (Cat. No. 8020) and ECs (Cat. No. 8000) were purchased from ScienCell.

Techniques: Expressing, Co-Culture Assay, Control

Journal: International Journal of Medical Sciences

Article Title: The phenotype of vascular smooth muscle cells co-cultured with endothelial cells is modulated by PDGFR-β/IQGAP1 signaling in LPS-induced intravascular injury

doi: 10.7150/ijms.34749

Figure Lengend Snippet: SMCs Shift phenotypic transition to synthetic type from contractile type orchestrated by ECs. (A) , LPS increased the migration ability of SMCs. When SMCs were co-cultured with ECs, the increased migration induced by LPS was alleviated. (A1) , Quantification of A is shown in A1, [% width of injury line = (a - b) × 100%/a; a = Initial scratch wound area at 0 h, b = Scratch wound area at 24 h] **: P<0.01, SMC Control vs SMC LPS, ##: P<0.01, CULSMC Control vs CULSMC LPS, @@: P<0.01, SMC LPS vs CULSMC LPS; (B) , LPS induced the reduction of SM22 and α-SMA in SMCs, however, when SMCs were co-cultured with ECs, the reduction of SM22 and α-SMA protein induced by LPS were partly alleviated. Meanwhile, ECs could enhance the expression of SM22 and α-SMA in SMCs in normal condition. (B1 and B2) . Quantification of B is shown in B1 and B2, **: P<0.01, SMC Control vs SMC LPS, ##: P<0.01, #: P<0.05, SMC LPS vs CULSMC LPS; CULSMC Control vs CULSMC LPS, @@: P<0.01, SMC LPS vs CULSMC LPS; &: P<0.05, SMC Control vs CULSMC Control.

Article Snippet: Human umbilical vein SMCs (Cat. No. 8020) and ECs (Cat. No. 8000) were purchased from ScienCell.

Techniques: Migration, Cell Culture, Control, Expressing

Journal: International Journal of Medical Sciences

Article Title: The phenotype of vascular smooth muscle cells co-cultured with endothelial cells is modulated by PDGFR-β/IQGAP1 signaling in LPS-induced intravascular injury

doi: 10.7150/ijms.34749

Figure Lengend Snippet: LPS induced a higher higher level of PDGFR-β and IQGAP1 in SMCs in both the single and co-culture systems. (A) , LPS induced the level of p-PDGFR-β expression in SMCs in both single and coculture system, but when SMCs were co-cultured with ECs, both control and LPS group experienced the relative lower p-PDGFR-β protein expression than that in single culture system. With LPS stimulation, p-PDGFR-β levels in co-culture SMCs were remarkably lower than those of single-culture SMCs. Quantification of A is shown in A1; **: P<0.01, SMC Control vs SMC LPS, #: P<0.05, CULSMC Control vs CULSMC LPS, @: P≤0.05, SMC LPS vs CULSMC LPS; (B) , the change of IQGAP1 expression was mediated by LPS, Quantification of B is shown in (B1) ; **: P<0.01, SMC Control vs SMC LPS, #: P<0.05, CULSMC Control vs CULSMC LPS, @@: P<0.01, SMC LPS vs CULSMC LPS.

Article Snippet: Human umbilical vein SMCs (Cat. No. 8020) and ECs (Cat. No. 8000) were purchased from ScienCell.

Techniques: Co-Culture Assay, Expressing, Cell Culture, Control